|The Effect of Methamphetamine on Oocyte Quality, Fertilization Rate and Embryo Development in Mice|
|Maryam Nezhad Sistani1, Marefat Ghaffari Novin1,2, Fatemeh Fadaee Fathabadi1, Mohamad Salehi2,3, Maryam Salimi1, Zahra Shams Mofarahe1, Mahdi Hadi4|
|1Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
IJWHR 2016; 4: 008-012
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Keywords : Cleavage, Embryonic development, Fertilization in vitro, Methamphetamine, Oocytes, Preimplantation
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Objective: Methamphetamine (METH) is an illicit psychoactive drug. There are different reasons of abusing METH such as have afun and to develop sexual satisfaction. This study was designed to investigate the effects of short and long term use of METH on oocyte and embryo development in mice.
Material and Method: In this study, 75 female NMRI mice were divided into five groups of 15. The groups consisted of a control group, experimental groups A and B, which received MA (10 mg/kg/day) intraperitoneally for 2 and 14 consecutive days, respectively and two sham groups A and B, which only received saline. After the last injection, 10 IU PMSG and 10 IU HCG were administered intraperitoneally for induction ovulation. Then, all the mice were sacrificed to aspirate their oocytes for further evaluation. In-vitro fertilization was done by using mature oocytes and embryo developmentwas investigated to the blastocyst stage. Data analyzed by using SPSS and Non parametric Mann-Whitney test.
Results: The number of fragmented oocytes in experimental group A was significantlyincreased in comparison with experimental group B and control group (P?0.05). Also there were significant differences in the number of unfertilized oocytes and early cleavage stage embryos in experimental group B in comparison with other groups that Indicates the reduction offertilization ratein experimental group B (P?0.05).
Conclusion: Short-termadministrationof METH in mice can affect oocyte quality but had no effect on early embryo development,whilethelong-term administration may effect on oocyte and embryo development in early cleavage stage.
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