Materials and Methods: This quasi-experimental study was conducted between 2024 and 2025. Immature germinal vesicle (GV)-stage oocytes (n=24) from patients with PCOS were allocated to either a non-PRP group (n=12 oocytes) cultured in standard G-IVF™ medium or a PRP group (n=12 oocytes) cultured in G-IVF™ supplemented with 5% allogeneic PRP. After 24 hours, GDF-9 and BMP-15 concentrations in the culture media were quantified using ELISA.

Results: Post-IVM, GDF-9 and BMP-15 levels were significantly higher in the PRP group (1.036 ± 0.004 pg/mL and 0.888 ± 0.008 pg/mL, respectively) than in the non-PRP group (1.032 ± 0.003 pg/mL and 0.878 ± 0.004 pg/mL) (P = 0.020 and P = 0.001, respectively). Multivariate analysis confirmed that PRP supplementation was the only significant variable associated with increased GDF-9 (B=0.004, P = 0.047, R²=33.7%) and BMP-15 (B=0.012, P = 0.002, R²=50.7%) levels. Although the absolute changes were small (ΔGDF-9 = 0.004 pg/mL; ΔBMP-15 = 0.012 pg/mL), large effect sizes were observed (Cohen’s d = 1.13 and 1.58).

Conclusions: Supplementing IVM medium with 5% allogeneic PRP was associated with increased GDF-9 and BMP-15 levels, which are considered surrogate biomarkers of oocyte maturation.