Methods and Materials: In this experimental study, 32 female rats were randomly divided into four groups: Group 1 (control), group 2 (OTD group, subjected to ovarian torsion/detorsion), group 3 (OTH group, torsion/detorsion + 50 mg/kg hesperidin), and group 4 (healthy group, receiving 50 mg/kg hesperidin without torsion). After 21 days of treatment, ovarian tissues were harvested for histological assessment, and mRNA levels of ER, FSHR, and LHCGR were analyzed. Serum concentrations of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estrogen were also measured.

Results: Histological analysis revealed that OTD induced structural damage, including oocyte and granulosa cell shrinkage, which was mitigated by hesperidin administration. The treated groups exhibited increased serum levels of estrogen, LH, and FSH, along with decreased testosterone levels. Additionally, hesperidin significantly upregulated the expression of ER, FSHR, and LHCGR, suggesting a positive effect on ovarian function and fertility.

Conclusions: The results indicate that hesperidin exerts protective effects on ovarian tissue following torsion/detorsion. It modulates hormonal balance and enhances the expression of key reproductive receptors (ER, FSHR, LHCGR), suggesting protective effects on ovarian function; however, further clinical studies are required before definitive conclusions can be drawn regarding its impact on fertility.