Materials and Methods: The MDA-MB-231 cell line was treated with different concentrations of three extracts from L. angustifolia. MTT assay was performed to evaluate cell viability, and flow cytometry was used to examine the cell cycle. The potential of the breast cancer cell line to form the colonies after a treatment with the plant extract was also investigated. The matrix metalloproteinase 2 and 9 (MMP2 and 9) were measured by quantitative real-time polymerase chain reaction (qPCR). Molecular docking was performed using AutoDock Vina in PyRx0.8 to investigate the likely target protein.

Results: The results showed that the methanol extract had considerable cytotoxicity to MDA-MB-231. The G0/G1 phase arrest was significantly increased in MDA-MB-231 after treatment with methanol extract. Furthermore, MMP2 and 9 significantly down-regulated the post treatment with the plant extract. The docking energy (kcal.mol-1) of eight methanolic extract compounds was docked with the histone deacetylase (HDAC), and nine transcription factors were achieved.

Conclusions: In sum, a cytotoxic property was determined for L. angustifolia methanol extract on the MDA-MB-231. According to the results from docking studies, the best compound among the investigated compounds was 2-furancarboxaldehyde, which was docked with HDAC with a –7.37 kcal.mol-1 docking score. Therefore, it was recommended that future studies should be carried out to further investigate L. angustifolia as a possible complementary treatment.