|The Effect of Different Concentrations of Calcium and Sodium on the Sperm Motility and Sex Determination in IVF Process|
|Akram Goudarzi1, Nafiseh Tavakolpoor Saleh2, Somayeh Karami3, Farima Mohammadian Amiri4, Parisa Bahavar5, Tohid Moradi Gardeshi6|
|1Department of Immunochemistry, Avicenna Research Institute, Tehran, Iran
2Department of Biophysics and Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4Clinical Research Development Unit of Rouhani Hospital, Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran
5Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran
6Department of Veterinary Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
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Keywords : Calcium, Sodium, Sperm motility, Sex determination
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Objectives: This study aimed to investigate the effect of a treatment with sodium and calcium bicarbonate on the sex determination of a fetus.
Materials and Methods: A total of 60 mice were obtained from Pasteur Institute, Karaj, Iran. Female mice were stimulated to ovulate with PMSG hormone, and the oocyte was released by human chorionic gonadotropin (hCG) hormone. Sperms were collected from the male mice. In vitro fertilization (IVF) was performed in the completely sterile conditions in a control culture medium with pH=7.4. Then six experimental groups, three experimental groups with sodium bicarbonate as well as three experimental groups and the embryos, were transferred from the multicellular stage (the blastocyst) to pseudopregnant mice.
Results: Three groups treated with sodium bicarbonate with different pHs were significantly different from the control group regarding the percentage of the born male fetuses (P ≤ 0.05); however, this difference was not significant between the sodium bicarbonate groups with different pH (P ≥ 0.05). Among the groups receiving calcium bicarbonate treatment, only the average pH level of male infants at 7.7 demonstrated a significant distinction when compared to both the control group and the remaining treatment groups (P ≤ 0.05).
Conclusions: Using sodium bicarbonate and calcium bicarbonate as well as adjusting the pH of the oocyte and sperm fertilization environment based on them may have been a safe and cheap method to determine the sex of a fetus.
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