|Chemotherapy-Related Structural Changes in Cancer: Effect of GnRH Antagonist in the Ovarian Follicles|
|Daryosh Mohammadnejad1, Faeze Daghigh2, Arezou Hamzehzadeh3|
|1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Physiology, Faculty of Medicine, Tabriz Medical sciences, Islamic Azad University, Tabriz, Iran
3Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran
IJWHR 2022; 10: 214-218
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Keywords : Chemotherapy, Gonadotropin-releasing hormone, Follicle-stimulating hormone, Luteinizing hormone, Thiotepa
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Objectives: The adverse effect of chemotherapy on the proliferation of granulosa cells has been indicated in recent studies. Gonadotropin-releasing hormone (GnRH) antagonists exert protective effects on granulosa cells against the side effects of chemotherapy. In the present study, we aimed to evaluate the impact of cetrorelix on the proliferation of ovarian granulosa cells following administration of thiotepa in the ovaries of female mice.
Materials and Methods: In this experimental study, 30 adult Balb/c female mice (5-8 weeks old, weighing 24-28 g) were divided into three groups (n=10/ each group) (Control group, T. group, and C. group). T. group received 2.5 mg/kg of thiotepa for four consecutive days. The C. group received cetrorelix (0.25 mg/kg) before and at the same time as thiotepa administration and a week after the end of thiotepa administration. Ovaries were used for quantitative and immunohistochemical studies at the end of the investigation.
Results: The mean numbers of follicles such as primordial, primary, secondary, and tertiary significantly decreased in the T. group than control group (P = 0.02). Cetrorelix treatment exerted a protective effect against thiotepa-induced damage by increasing the mean numbers of follicles in the ovarian cortex (P = 0.04).
Conclusions: As a GnRH antagonist, cetrorelix can be considered as one of the effective drugs to protect the granulosa cells against chemotherapy-induced damages in cancer disease.
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