International Journal of Women's Health and Reproduction Sciences

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Apr 2020, Vol 8, Issue 2

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Study Protocol
Induction of the Mice Model Regarding the Endometriosis and Assessment of Antioxidant Treatment Effectiveness: An Experimental Protocol
Hayedeh Hoorsan¹, Masoumeh Simbar², Fahimeh Ramezani Tehrani³, Fardin Fathi⁴, Nariman Mosaffa⁵, Hedyeh Riazi⁶
¹Student Research Committee, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran ²Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran ³Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ⁴Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran ⁵Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran ⁶Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
IJWHR 2020; 8: 133-141 DOI: 10.15296/ijwhr.2020.21 Viewed : 2466 times Downloaded : 2367 times. Keywords : Model of endometriosis, Mice, First-generation, Antioxidant treatment
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Abstract

Objectives: Over the past twenty years, the role of inflammatory, immunologic, and stress oxidative factors in the pathogenesis of endometriosis has been emphasized in the literature. Endometrial induced lesions in the surgical endometriosis model of mice are similar to those observed in women. Therefore, the present study evaluated the effects of vitamin C treatment on the endometriosis mice model. More precisely, it aimed to induce an optimum model of endometriosis using both peritoneal and ovarian approaches and to compare the histological and fertility changes in the first-generation infants of endometriosis induced, sham, and control groups. Finally, the study assessed the influence of vitamin C administration treatment on the induced endometriosis mice model and its first-generation infants. Methods: This experimental study was done in three phases. In the first phase, 32 mice were randomly divided into four groups receiving induced endometriosis through peritoneal approach, induced endometriosis through an ovarian approach, as well as sham and control groups. The histological and fertility characteristics of the first generation of ovarian endometriosis, sham, and control groups were compared in the second phase. Then, the histological and fertility characteristics of those endometriosis induced models receiving vitamin C were compared with the controls, and the first generation of these groups was compared as well. Results: This experimental study began in April 2017 and will be finished in 2020. Conclusions: The results of this study improve our knowledge about the mechanisms involved in endometriosis progressions. The results further provide a comprehensive animal model for further research and assess the therapeutic role of antioxidants.