International Journal of Women's Health and Reproduction Sciences

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Jul 2019, Vol 7, Issue 3

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Original Article
The Inhibitory Effect of Hsa-miR-330 Replacement on the Proliferation and Migration of Breast Cancer MCF-7 Cells
Elham Hosseinzadeh^1,2, Behzad Mansoori^1,3, Ali Mohammadi¹, Vahid Khaze¹, Maryam Rezazadeh², Behzad Baradaran¹
¹Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ²Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran ³Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
IJWHR 2019; 7: 360?365 DOI: 10.15296/ijwhr.2019.59 Viewed : 3640 times Downloaded : 2654 times. Keywords : Breast cancer, E2F1, Hsa-miR-330, Cancer therapy
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Abstract

Objectives: miRNAs comprise a group of master gene expression regulators, exerting their effects after transcription through targeting specific mRNAs, hence, influencing cellular processes. A considerable number of miRNAs are known to affect cell proliferation and migration in breast cancer, one of which is hsa-miR-330, a key player in various types of cancers. The purpose of this study was to evaluate the anti-proliferative and anti-migrative effects of hsa-miR-330 on MCF-7 cell line. Materials and Methods: MCF-7 cells were transfected with pCMV-miR-330 vector and cell selection was performed in media containing Geneticin (G418). Subsequently, MTT assay was performed to evaluate the effect of hsa-miR-330 on proliferation and scratch wound healing assay was employed to evaluate cellular migration. Finally, using real-time PCR, the expression of hsa-miR-330 as well as the repressive impact on the expression of E2F was investigated. Results: Upon confirmation of hsa-miR-330 induction in MCF-7 cells via GFP channel imaging system, miR-330 expression was demonstrated to be increased 10 folds in stable cells. The results of MTT and wound-healing assays demonstrated an inhibitory role for hsa-miR-330 in proliferation and migration of stable hsa-miR-330-transfected MCF-7 cancer cells compared to controls. In addition, after transfecting cells with hsa-miR-330, E2F1 was down-regulated in comparison with controls. Conclusions: Based on the results of the current study, we suggest a potential inhibitory role for hsa-miRNA-330 in cell proliferation as well as cell migration in breast cancer by targeting E2F1 mRNA. Additionally, a therapeutic role can be suggested for hsa-miR-330 in terms of target therapy for breast cancer.