International Journal of Women's Health and Reproduction Sciences

Home Aim and Scope Instructions to Authors Editorial Policies Editorial Board Authorship Agreement Form Open Access and Copyright

Mar 2014, Vol 2, Issue 2

CURRENT ISSUE ARCHIVE AHEAD OF PRINT / ACCEPTED ARTICLE MOST VIEWED ARTICLES NEWS RELATED LINKS

Advanced Search

For Author's & Reviewer's

Committee on Publication
Ethics (COPE) Flowchart

Recommendations for the Conduct

COPE: Reviewers

World Association of Medical Editors(WAME)

EQUATOR Resources : Reviewers

Poll

How do you find the scientific quality of the published articles on our web site?

Very good

Good

Fair

Bad

Very bad

Original Article
Detection of Acute Toxoplasmosis: The Genitally Transmittable Infection
Saeedeh Shojaee, Hossien Keshavarz
Tehran University of Medical Sciences, School of Public Health, Tehran, Iran
IJWHR 2014; 2: 090-093 DOI: 10.15296/ijwhr.2014.13 Viewed : 3706 times Downloaded : 3214 times. Keywords : Antigenemia, PCR, Toxoplasma
Full Text(PDF) \| Related Articles
Abstract

Objectives: Toxoplasma gondii is an obligate intracellular parasite that infects a broad range of warm- blooded animals including human. Tachyzoites of T.gondii invade the host cell, replicate and finally lead to the lysis of the cell. T. gondii is associated with congenital infection and it can cause encephalitis, or systemic infection in immunocompromised patients. It is important to know whether the infection is recently acquired or is chronic. Differentiation between acute and chronic infection has a dramatic impact, especially for the developing fetus. In this study, Toxoplasma gondii was detected in acute phase of infection in serum sample of a person who had been accidentally infected with tachyzoites of RH strain in the laboratory. Materials and Methods: Anti- T.gondii IgG antibody was prepared by rabbit immunization with soluble antigen of tachyzoites of RH strain. Capture- ELISA, immunoblotting and PCR were performed in the laboratory. Results: Antigenemia and parasitemia was detected in serum sample of infected person by capture_ELISA, immunoblotting and PCR techniques respectively. Conclusion: Acute T.gondii infection could be detectable in a short period of time in the sera of infected person.