|Relationship Between Follicular Fluid and Serum Levels of Vitamin C and Oocyte Morphology and Embryo Quality in Patients Undergoing In Vitro Fertilization|
|Seyedeh Hajar Sharami1, Mohammad Hadi Bahadori2, Fereshteh Fakor1, Fariba Mirblouk1, Sodabeh Kazemi1, Davood Pourmarzi2, Safoora Saffari3, Seyedeh Fatemeh Dalil Heirati1|
|1Reproductive Health Research Center, Department of Obstetrics & Gynecology, Azzahra Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2Department of Obstetric and Gynecology, Guilan University of Medical Sciences, Rasht, Iran.
3Department of developmental Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
IJWHR 2017; 5: 41-46
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Keywords : Embryo quality, Follicular fluid, IVF, Oocyte morphology, Vitamin C
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Objective: This study aimed to determine the relationship between follicular fluid (FF) and serum level of vitamin C and oocytes morphology and embryo quality in patients undergoing in vitro fertilization (IVF).
Materials and Methods: This is a cross-sectional study which was conducted on 50 women undergoing IVF in Al-Zahra hospital in Rasht, Iran during 2014. Vitamin C level in FF and serum were measured using high-performance liquid chromatography (HPLC). Retrieval oocyte morphology and obtained embryo quality were evaluated using inverted optical microscope.
Results:In our study 434 oocytes and 199 embryos were assessed. Frequencies of metaphase II (MII) oocytes were significantly higher at 1-1.5 mg/dL level of vitamin C in FF (86.4%) and 0.5-1 mg/dL serum level of vitamin C (83.2%). Odds of having MII oocytes at the 1-1.5 mg/dL level of vitamin C in FF (odds ratio [OR] = 15.02, 95% CI = 2.77-81.54) was 15 times greater than the level of 1.5-2.3 mg/dL. The most frequency of embryo with Z1 or Z2 quality were observed at 0.1-0.5 mg/dL level of vitamin C in FF (69.3%) and 0.5-1 mg/dL level of vitamin C in serum (84.3%). odds of having embryo with Z1 or Z2 quality, at the 0.1-0.5 mg/dL level of vitamin C in FF (OR = 3.08, 95% CI = 1.21-7.83), was 3 times greater compared to the 1-2.3 mg/dL level.
Conclusion: At the certain level of vitamin C in FF, clinicians may have oocytes with higher maturity and embryos with better quality.
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