Material and Method: In this study, 75 female NMRI mice were divided into five groups of 15. The groups consisted of a control group, experimental groups A and B, which received MA (10 mg/kg/day) intraperitoneally for 2 and 14 consecutive days, respectively and two sham groups A and B, which only received saline. After the last injection, 10 IU PMSG and 10 IU HCG were administered intraperitoneally for induction ovulation. Then, all the mice were sacrificed to aspirate their oocytes for further evaluation. In-vitro fertilization was done by using mature oocytes and embryo developmentwas investigated to the blastocyst stage. Data analyzed by using SPSS and Non parametric Mann-Whitney test.

Results: The number of fragmented oocytes in experimental group A was significantlyincreased in comparison with experimental group B and control group (P?0.05). Also there were significant differences in the number of unfertilized oocytes and early cleavage stage embryos in experimental group B in comparison with other groups that Indicates the reduction offertilization ratein experimental group B (P?0.05).

Conclusion: Short-termadministrationof METH in mice can affect oocyte quality but had no effect on early embryo development,whilethelong-term administration may effect on oocyte and embryo development in early cleavage stage.