|Prevalence and Genotype Distribution of Human Papillomavirus Among a Subpopulation of Jordanian Women|
|Ashraf I. Khasawneh1, Fida F.Asali2, Rami M.Kilani2, Jumana A.Abu-Raideh1, Nisreen M. Himsawi1, Muna A. Salameh3, Ghadeer H.Al Ghabbiesh4, Tareq Saleh1|
|1Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, Zarqa, Jordan
2Department of Obstetrics and Gynecology, Faculty of Medicine, The Hashemite University, Zarqa, Jordan
3Department of Basic Medical Sciences, College of Medicine, Al-Balqa' Applied University, Al-Salt, Jordan
4Molecular Biology Department, MedLabs Consultancy Group, Amman, Jordan
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Keywords : Human papillomavirus, Real-time PCR, HPV 16, Prevalence; Jordan, Cervical cancer
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Objectives: Human papillomavirus (HPV) infection is the leading sexually-transmitted infection worldwide and the main etiology of cervical cancer. HPV infection rates are important in directing vaccination policies and screening for cervical cancer. Unfortunately, no recent reports have evaluated the prevalence of HPV infection amongst Jordanian women, despite the major globalization-driven changes in sexual behavior. This study aims to determine the prevalence of HPV infection and its genotypic distribution in cervical samples of Jordanian women.
Materials and Methods: The study was carried out at Prince Hamza Hospital (Amman, Jordan) during 2016-17. Women (15 and 75 years old) were randomly selected for cervical cell collection. DNA was extracted and then amplified using MY09/11 and GP5+/6+ consensus primers. Positive samples were then genotyped using RT-PCR and reverse line blotting.
Results: Fourteen out of 348 women tested positive for HPV with a prevalence rate of 4%. Multiple HPV genotypes were seen in 36% (5/14) of infected women, while single HPV genotype infection was seen in 64% (9/14) of infected women. High-risk HPV genotypes were detected in most cases with 78.6% (11/14) infection rate, potential high-risk HPV genotypes were detected in 42.9% (6/14), and low-risk HPV genotypes were detected in 7.1% (1/14) of infected women. Ten different genotypes were detected in the infected women; the most common type was HPV 16 (42.9%; 6/14).
Conclusions: Our data suggests that the prevalence of HPV infection amongst Jordanian women is below the global and regional rates. This data should facilitate the implementation of appropriate cervical cancer screening and future HPV vaccination programs.
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