|Curcumin Delay Oocytes Apoptosis by Overexpression of BCL-2 gen in Young and Middle-Aged Mice Model|
|Saeideh Hasani Azami1, Hamid Nazarian1, Mohammad-Amin Abdollahifar1, Mehdi Allahbakhshian-Farsani3, Seyede Zahra Banihosseini1, Marefat Ghaffari Novin1,2|
|1Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Department of Hematology and Blood Banking, Faculty of Allied Medicin Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Keywords : Aging; Apoptosis, Curcumin, Oocyte, Oxidative stress
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Objectives: We determined the effects of curcumin as a well-known antioxidant on prevention apoptosis of mature oocytes during the natural increasing age in female mice. Oxidative stress could initiate the process of apoptosis which affects the oocytes quality and reduces development competency in the ovarian follicles.
Materials and Methods: This study is based on case-control, interventional, quantitative applied research. 21-day-old NMRI female mice were used as a control, vehicle, and curcumin groups. The mice in curcumin groups received 100 mg/kg/day curcumin intraperitoneally. After initial interventions, the Annexin-V- FLUOS staining was applied to evolution the oocytes apoptosis rate in the three groups at 6, 12, and 33 weeks of age. The expression of oocytes apoptosis-related genes (Bcl2 and Bax) was also assessed by real-time PCR technique and oxidation-reduction markers were measured in the ovaries.
Results: Our results showed that oocytes apoptosis and necrosis in curcumin groups were decreased in comparison with the control and vehicle groups at 12 and 33 weeks (p<0.001). Moreover, the use of curcumin led to upregulation of Bcl2 and downregulation of Bax genes at 6,12 and 33 weeks (p<0.001). Also, the superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity were increased in curcumin groups compare with control and vehicle groups at 12 and 33 weeks (p<0.001) however, malondialdehyde (MDA) was decreased in the curcumin group at control 12 (p<0.001), vehicle 12 (p<0.01) and 33 weeks (p<0.01).
Conclusion: Thus curcumin could suppress oocytes apoptosis through upregulation of Bcl2 and downregulation of Bax gene as well as by suppressing oxidative stress pathways involving oocytes apoptosis and necrosis.
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