|Hsa-miR-330 Replacement Inhibits Breast Cancer MCF-7 Cell Proliferation and Migration|
|Elham Hosseinzadeh1,2, Behzad Mansoori1,3, Ali Mohammadi1, Vahid Khaze1, Maryam Rezazadeh2 , Behzad Baradaran1|
|1Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Medical genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Breast cancer, E2F1, hsa-miR-330, Cancer therapy
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Objectives: miRNAs comprise a group of master gene expression regulators, exerting their effect after transcription through targeting specific mRNAs, hence, influencing cellular processes. A considerable number of miRNAs are known to affect cell proliferation and migration in breast cancer, one of which is hsa-miR-330, a reportedly key player in various types of cancers. The purpose of this study was to evaluate hsa-miR-330 in terms of the anti-proliferative and anti-migrative contribution in MCF-7 cell line.
Materials and Methods: MCF-7 cells were transfected with pCMV-miR-330 vector and cell selection was performed in media containing Genetic in (G418). Subsequently, MTT assay was performed to evaluate the effect of hsa-miR-330 on proliferation, and scratch wound healing assay was employed to evaluate cellular migration. Finally, using Real-Time PCR, the expression of hsa-miR-330 as well as the repressive impact on the expression of E2F was investigated.
Results:Upon confirmation of hsa-miR-330 induction in MCF-7 cells via GFP channel imaging system, miR-330 expression was demonstrated to be increased 10 fold in stable cells. The results of MTT and wound healing assays demonstrated an inhibitory role for hsa-miR-330 on proliferation and migration of stable hsa-miR-330-transfected MCF-7 cancer cells compared to controls.In addition, after transfecting cells with hsa-miR-330,E2F1 was down-regulated in comparison with controls.
Conclusions:Based on the results of the current study, we suggest a potential inhibitory role for hsa-miRNA-330 on cell proliferation as well as cell migration in breast cancer by targeting E2F1 mRNA. Additionally, a therapeutic role can be suggested forhsa-miR-330 in terms of target therapy of breast cancer.
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