|The Effect of Implant Origin Differences on Peritoneal Endometriosis in An Endometriosis Mouse Model|
|Sutrisno, Andarini S.1, Wiyasa IWA1, Kulsum U.1, Noerhamdani1, Suyuti H1, Hendarto H1|
|Department of Obstetric and Gynecology, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia|
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Keywords : Endometriosis, Granuloma mass, Implant area, Peritoneal damage
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Objectives: This study aims to observe the difference in the area of endometriosis lesions and the histopathology of inflammatory cells and granuloma masses in an endometriosis mouse model treated with endometrial cell implants, endometrioma capsules, and adenomyosis tissue.
Materials and Methods: This an experimental study with Post-Test Research Design Only with Control Group Design. Thirty-two mice (Mus musculus) were injected with 0.2 mL/mice cyclosporin A. Mice were divided into 3 groups: Group A was injected with endometrial tissue from the uterine cavity; Group B was injected with endometriosis from endometrioma capsule, and Group C was injected with endometriosis from adenomyosis. The injection was done slowly into the peritoneal cavity, 0.1 mL each, and followed by intramuscularly Ethinyl estradiol, 0.2 μg/mice. On the 15th days, mice were dissected to observe the peritoneal endometriosis implant and microscopic examination with HE staining to determine the inflammatory cell infiltration and mass granuloma presence. Data were analysized using SPSS for Windows 19.0.
Results: The study obtained that the area of implanted endometriosis lesions in Group C covered a larger area of endometriosis implants than other groups (p < 0.05). The peritoneal damage in Group C was the most severe based on the Klopfleisch method (p < 0.05), with mass granuloma and massive infiltration of inflammatory cells and fibrous connective tissue formation occurring in muscle tissue.
Conclusions: The implantation of adenomyosis cell tissue is the best method to develop mice model of endometriosis based on its inflammatory infiltration, the extent of lesion implant, and granuloma mass.
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