Login Contact Us
Jan 2019, Vol 7, Issue 1
Advanced Search
How do you find the scientific quality of the published articles on our web site?
Original Article
The Effect of Implant Origin Differences on Peritoneal Endometriosis in An Endometriosis Mouse Model
Sutrisno, Andarini S.1, Wiyasa IWA1, Kulsum U.1, Noerhamdani1, Suyuti H1, Hendarto H1
Department of Obstetric and Gynecology, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia

Viewed : 154 times
Downloaded : times.

Keywords : Endometriosis, Granuloma mass, Implant area, Peritoneal damage
| Related Articles

Objectives: This study aims to observe the difference in the area of endometriosis lesions and the histopathology of inflammatory cells and granuloma masses in an endometriosis mouse model treated with endometrial cell implants, endometrioma capsules, and adenomyosis tissue.

Materials and Methods: This an experimental study with Post-Test Research Design Only with Control Group Design. Thirty-two mice (Mus musculus) were injected with 0.2 mL/mice cyclosporin A. Mice were divided into 3 groups: Group A was injected with endometrial tissue from the uterine cavity; Group B was injected with endometriosis from endometrioma capsule, and Group C was injected with endometriosis from adenomyosis. The injection was done slowly into the peritoneal cavity, 0.1 mL each, and followed by intramuscularly Ethinyl estradiol, 0.2 μg/mice. On the 15th days, mice were dissected to observe the peritoneal endometriosis implant and microscopic examination with HE staining to determine the inflammatory cell infiltration and mass granuloma presence. Data were analysized using SPSS for Windows 19.0.

Results: The study obtained that the area of implanted endometriosis lesions in Group C covered a larger area of endometriosis implants than other groups (p < 0.05). The peritoneal damage in Group C was the most severe based on the Klopfleisch method (p < 0.05), with mass granuloma and massive infiltration of inflammatory cells and fibrous connective tissue formation occurring in muscle tissue.

Conclusions: The implantation of adenomyosis cell tissue is the best method to develop mice model of endometriosis based on its inflammatory infiltration, the extent of lesion implant, and granuloma mass.

Cite By, Google Scholar

Google Scholar

Articles by Sutrisno S
Articles by S. A
Articles by Iwa W
Articles by U. K
Articles by Noerhamdani N
Articles by H S
Articles by H H


Articles by Sutrisno
Articles by Andarini S.
Articles by Wiyasa IWA
Articles by Kulsum U.
Articles by Noerhamdani
Articles by Suyuti H
Articles by Hendarto H

Submit Paper
Online Submission System
IJWHR ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Published Article Statistics