|The Effect of Galega Officinalis on Hormonal and Metabolic Profile In A Rat Model of Polycystic Ovary Syndrome (PCOS)|
|Seyed Hosein Abtahi-Eivari1, Maryam Moghimian1, Malihe Soltani1, Hamed Shoorei2, Reza Asghari2, Hossein Hajizadeh3, Majid Shokoohi2,4, Somayye Alami1, Faezeh Khalife Ghaderi5|
|1Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
2Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Nutrition, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4Student in nursing, Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran
5Department of Midwifery, Faculty of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran
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Keywords : Galega Officinalis; PCOS; Steroid hormones; Rat
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Objectives: Among endocrine diseases in females in their regenerative age, polycystic ovary syndrome (PCOS) has been considered as one of the most themwith a prevalencerange of 5 to 21%. However, the purpose of the present study was to investigate the protective effect of G. Officinalis on metabolic as well as hormonal parameters in a rodent model of PCOS.
Materials and Methods: Thirty-two Wistar female rats were used (n=8/group) in thestudy consisting ofhealthy control and experimental groups. The experimental groups were divided into 3 subgroups, including rats with PCOS which were received no treatment(G1),PCOS group which was daily treated with G. Officinalis extract 200 mg/kg/orally (G2) for 2 weeks, and PCOS groupwhich wasdaily treated with G. Officinalis extract 400 mg/kg/orally (G3) for 2 weeks. In all experimental groups, a single injection of estradiol valerate intramuscularly led to inducing PCOS. Afterthe end of treatment period, ratsin all of the studied groupsanesthetized with ketamine/xylazine (5/1 mg/kg), then the blood samples obtainedand their serum samples wereappliedfor testing the fasting blood glucose (FBS), insulin, aromatase, and hormones—follicle-stimulating hormone (FSH),luteinizing hormone (LH),testosterone, and estrogen.The ovaries of ratswere removed and fixed for histopathological examinations.
Results: The serum levels of FBS, insulin, LH, FSH, and testosterone significantly increased in G1in comparison tohealthy rats(P<0.05), while they were all significantly decreased in the treated groups received G. Officinalis extract compared to rats affected by PCOS(P<0.05). Moreover, the serum level of estrogen and the serum activity of aromatase were both significantly decreased in G1in comparison tohealthy rats(P<0.05), while in treated groups, they were significantly increasedcomparedtoG1received no treatment (P<0.05). Moreover, the number of follicles in ovaries affected by PCOS decreased, whileboth concentrations of G. Officinalis extract could prevent this phenomenon.
Conclusion: It seems that the extract of Galega Officinalis has a beneficial effect on the levels of the LH, FSH, testosterone, estradiol, aromatase, FBS, and insulin in alleviation of PCOS complications.
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