Login Contact Us
Mar 2014, Vol 2, Issue 2
Advanced Search
Title
Authors
Keyword
Poll
How do you find the scientific quality of the published articles on our web site?
Original Article
Detection of Acute Toxoplasmosis: The Genitally Transmittable Infection
Saeedeh Shojaee, Hossien Keshavarz
Tehran University of Medical Sciences, School of Public Health, Tehran, Iran

IJWHR 2014; 2: 090-093
DOI: 10.15296/ijwhr.2014.13
Viewed : 2067 times
Downloaded : 1947 times.

Keywords : Antigenemia, PCR, Toxoplasma
Full Text(PDF) | Related Articles
Abstract
Objectives: Toxoplasma gondii is an obligate intracellular parasite that infects a broad range of warm- blooded animals including human. Tachyzoites of T.gondii invade the host cell, replicate and finally lead to the lysis of the cell. T. gondii is associated with congenital infection and it can cause encephalitis, or systemic infection in immunocompromised patients. It is important to know whether the infection is recently acquired or is chronic. Differentiation between acute and chronic infection has a dramatic impact, especially for the developing fetus. In this study, Toxoplasma gondii was detected in acute phase of infection in serum sample of a person who had been accidentally infected with tachyzoites of RH strain in the laboratory.

Materials and Methods: Anti- T.gondii IgG antibody was prepared by rabbit immunization with soluble antigen of tachyzoites of RH strain. Capture- ELISA, immunoblotting and PCR were performed in the laboratory.

Results: Antigenemia and parasitemia was detected in serum sample of infected person by capture_ELISA, immunoblotting and PCR techniques respectively.

Conclusion: Acute T.gondii infection could be detectable in a short period of time in the sera of infected person.

Cite By, Google Scholar

Google Scholar

Articles by Shojaee S
Articles by Keshavarz H

PubMed

Articles by Saeedeh Shojaee
Articles by Hossien Keshavarz

Submit Paper
Online Submission System
IJWHR ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Published Article Statistics