|Superovulation Affects the Gene Expression Patterns of Mice Oocytes and Preimplantation Embryos Produced by Different Assisted Reproductive Technologies|
|Elhameh Jahanbakhsh-Asl1, Mohammad Salehi2,3, Marefat Ghaffari-Novin1, Yoko Kato4|
|1Department of Biology and Anatomical Science, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
2Department of Biology and Anatomical Science, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
3Department of Biotechnology, School of Advanced Technologies in Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
4Laboratory of Animal Reproductive, College of Agriculture, KINDAI University, Nara, Japan
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Keywords : ART, Epigenetics, Gonadotrophins, Superovulation
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Objectives: In this experiment, we examined whether superovulation could affect important molecular profiles of oocytes and embryos in mice with regard to their development.
Materials and Methods: A total of 120 metaphase II oocytes were produced by super- or spontaneous ovulation. Blastocyst-stage embryos were produced after in vitro culture of oocytes fertilized by intra-cytoplasmic sperm injection (ICSI), in-vitro fertilization (IVF) and in vivo fertilization. Blastocysts obtained from naturally mated female without gonadotrophin treatment were also used as controls.Using real time PCR, the mRNA expression patterns of Bone morphogenesis protein (Bmp15(, Hepatoma-derived growth factor (Hdgf),DNA methyl transpherase-1(Dnmt-1), Developmental pluripotency associated 3(Dppa3), and Zinc finger protein 57 (Zfp57)genes were compared between superovulated and spontaneously ovulated oocytes and the expression patterns of H19 and Small Nuclear Ribonucleoprotein N)Snrpn(imprintinggenes between embryos produced from these oocyteswere also compared.
Results: Expression of five maternal effect genes(Bmp15, Hdgf,Dnmt-1, Dppa3, and Zfp57) in superovulated oocytes were significantly reduced (P < 0.05). The rates of cleavage to the blastocyst stages were not significantly different (P < 0.05) between superovulated and naturally ovulated oocytes in cultured embryos. Moreover, superovulation disturbed the expression patterns ofimprinting genes (H19 and Snrpn)in single blastocysts obtained by differentAssisted Reproductive Techniques(ART).
Conclusions: It was shown that superovulation affected the molecular characteristics of ART conceived embryos.
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