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Jul 2018, Vol 6, Issue 3
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Original Article
Immunohistochemical localization of Endothelial Nitric Oxide Synthase in Endometrial Tissues of Infertile Women with Uterine Myomas
Tohid Najafi1, Marefat Ghaffari Novin2, Ashraf Moini3, Niknezhad Sogol2
1Wayne State University, Biochemistry, Detroit, USA
2Department of Biology and Anatomical Science, Shahid Beheshti University of Medical Science, Tehran, Iran
3Department of Obstetrics and Gynecology,Tehran University of Medical Science, Tehran, Iran

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Keywords : Nitric oxide, Endothelial Nitric Oxide Synthase (eNOS), Endometrium, Uterine Myomas, Fibroids
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Objectives: Nitric oxide (NO) is a free radical that plays important roles in many physiological processes of the female reproductive system. Pathophysiological studies revealed a possible role of the endothelial isoform of nitric oxide synthase (eNOS) enzyme in female reproductive disorders specifically in the endometrium. This study investigates the expression of endothelial nitric oxide synthase in the endometrial tissue to study the potential role of this enzyme and its NO production in infertility of women with uterine myomas.

Materials and Methods: A total of 20 endometrial tissues were obtained, 10 infertile women with uterine fibroids and 10 from normal and fertile women, 7 to 9 days after LH surge. Following fixation with paraformaldhyde, frozen sections of samples were prepared for semi-quantitative immunohistochemical evaluation using monoclonal anti-human eNOS antibody. Hematoxilin and Eosin staining was used for histological dating of the samples.

Results: Localization of endothelial nitric oxide synthase was seen in glandular and luminal epithelium, vascular endothelium and stroma in both fertile women and infertile women with uterine fibroids. Although we found differences in immunoreactivity of luminal epithelium (p=0.105), vascular endothelium (p=0.143) and stroma (p=0.796) in both groups, over-expression of eNOS in glandular epithelium (p=0.015) was statistically significant in women with uterine fibroids compared to the control group.

Conclusion: This study suggests that over-expression of eNOS in glandular epithelium may influence the preparation stage of endometrium for fertility in women with uterine myomas.

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